Established from the lymph node of a 5-year-old Caucasian boy with B cell lymphoblastic non-Hodgkin lymphoma at diagnosis in 1994
Human hyperdiploid karyotype with 12% polyploidy - 48<2n>XYY, +21, ?ins(2;2)(q21;p15p22), t(8;14)(q24;q32)
round cells growing singly or in clusters in suspension
Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 -, CD138 -, sm/c
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 6 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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