Established from the bone marrow of a 28-year-old man who developed the terminal leukemic phase of lymphosarcoma in 1976
Human flat-moded hypertetraploid karyotype - 90-102<4n>XXY/XXYY, -3, -9, -12, +13, +14, +14, -15, +20, +20, +21, +21, +2-4mar, der(X;1)(p10;p10)/add(1)(q11), add(1)(p11), add(5)(p1?5), del(2)(p22p24), del(12)(q24.1), dup(21)(q11qter)x3-4 - resembles publi
single cells growing in suspension
Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture, PCR assays
Immunology: CD2 +, CD3 +, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta (+), TCRgamma/delta -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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