Established from the larvae of a tiger moth (species Trichoplusia ni = Autographa brassicae = Plusia ni, family Noctuidae, subfamily Plusiinae, order Lepidoptera)
spindle-shaped cells growing in suspension (90%); cells tend to cluster in aggregates
80% TC 100 medium + 20% h.i. FBS
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses:PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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