This cell line is a subclone of the original cell line R1 established after elimination of mycoplasma with BM-Cyclin (tiamulin & minocycline)
adherent, fibroblastoid cells growing as monolayers
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization and PCR assays
Viruses: PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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