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SK-GT-2

  • Specification
  • Recommended Products
Cat.No.
CSC-C0682
Description
Established in 1989 from the primary tumor of a 72-year-old Hispanic man with a poorly differentiated adenocarcinoma of the gastric fundus; cells were described to be noninvasive using a Boyden chamber assay
Species
Human
Source
gastric fundus carcinoma
Recommended Medium
Culture Properties
monolayer
Morphology
epithelial cells growing adherently as monolayer
Quality Control
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Viruses: PCR:
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

  • Q & A
  • Customer Review
Q:
How to remove fibroblasts using repeated apposition method during purification of tumor cells?
A:

Take three culture flasks A, B and C, first inoculate A with serum-free medium suspension of two kinds of cells, then gently aspirate the supernatant after 5-20 min and inoculate to B. Add complete medium to A and continue to culture; after culturing cells in B for 5-20 min, inoculate the supernatant to C in the same way, and then add complete medium to C. When the three bottles of cells are observed, it will be found that fibroblasts A>B>C, and the C bottle is mainly tumor cells, which can be processed repeatedly if purification is needed.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

Great

I purchased the cell product from Creative Bioarray and it met the requirements of my experiment. After that, I introduced this product to my friends.

13 July 2022


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