Established in 1989 from the primary tumor of a 72-year-old Hispanic man with a poorly differentiated adenocarcinoma of the gastric fundus; cells were described to be noninvasive using a Boyden chamber assay
gastric fundus carcinoma
epithelial cells growing adherently as monolayer
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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