These cells were thought to be "established from the malignant urinary bladder carcinoma of a 82-year-old Caucasian woman (stage pTa G1-G2) in 1992"; same donor as for cell line SBC-7; however, DNA fingerprinting and cytogenetic analysis at the Creative Bioarray established unequivocally that this cell line must be considered de facto a subclone of HELA
Human hypertriploid karyotype with 2% polyploidy - 75(66-80)<3n>XX, -X, +1, +3, -4, +5, +6, +8, -9, +10, +11, +11, +12, -13, -14, -15, +16, +17, -18, -22, +3-5mar, del(3)(q11), der(3)t(1;3)(q11;q13), der(3)t(3;5)(q13;q11), i(5p), i(7p)x1-2, der(8)t(8;11)(
epitheloid cells growing adherently in monolayers, often triangle-formed cells
Mycoplasma: contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, desmin -, end
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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