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SBC-2

Cat.No.: CSC-C0335

Species: Human

Source: cervix carcinoma

Morphology: epitheloid cells growing adherently in monolayers, often triangle-formed cells

Culture Properties: monolayer

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Cat.No.
CSC-C0335
Description
These cells were thought to be "established from the malignant urinary bladder carcinoma of a 82-year-old Caucasian woman (stage pTa G1-G2) in 1992"; same donor as for cell line SBC-7; however, DNA fingerprinting and cytogenetic analysis at the Creative Bioarray established unequivocally that this cell line must be considered de facto a subclone of HELA
Species
Human
Source
cervix carcinoma
Recommended Medium
90% RPMI-1640 + 10% h.i. FBS
Culture Properties
monolayer
Morphology
epitheloid cells growing adherently in monolayers, often triangle-formed cells
Karyotype
Human hypertriploid karyotype with 2% polyploidy - 75(66-80)<3n>XX, -X, +1, +3, -4, +5, +6, +8, -9, +10, +11, +11, +12, -13, -14, -15, +16, +17, -18, -22, +3-5mar, del(3)(q11), der(3)t(1;3)(q11;q13), der(3)t(3;5)(q13;q11), i(5p), i(7p)x1-2, der(8)t(8;11)(
Quality Control
Mycoplasma: contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, desmin -, end
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
What is the growth condition of the cells?

The cells are grown in suspension as aggregates and only the cells in the aggregates are viable, but cell viability cannot be estimated and the general medium contains a large amount of cellular debris.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

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We have been working with cells for many years and trust Creative Bioarray's products.

14 Feb 2022


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