KB-3-1

Cat.No.: CSC-C6211X

Species: Homo sapiens (Human)

Source: Uterus; Cervix

Morphology: epithelial-like, adherent cell line growing in monolayers

Culture Properties: monolayer

Inquire for Price

Specification
Background
Scientific Data
Q & A
Customer Review

Cat.No.

CSC-C6211X

Description

The parental continuous cell line HELA was established from the primary cervix carinoma of a 31-year-old black woman 1951; KB-3-1 is a derivative of HELA and is used in multiple drug resistance studies giving raise to drug-resistant mutants (e.g. KB-V1 resistant to vinblastine; KB-CHR-8-5 resistant to colchicine); cells express MYC mRNA; the cell line will only be distributed to non-profit institutions, commercial entities may apply for a license at the NIH/NCI Technology Transfer Cernter, Rockville, MD, USA, phone (301)435-3111

Species

Homo sapiens (Human)

Source

Uterus; Cervix

Recommended Medium

90% DMEM + 10% h.i.FBS + 1 mM sodium pyruvate

Culture Properties

monolayer

Morphology

epithelial-like, adherent cell line growing in monolayers

Karyotype

Human hypertriploid karyotype with 1.8% polyploidy - 76(73-78)<3n>XX, -X, -1, -3, -4, +6, +7, +8, -9, -18, -20, +11mar, der(1)t(1;?)((p11;?), der(3)t(3;?)(p11;?), der(3) t(3;?)(p14;?), der(3p;5q), del(6)(q21), del(7)(q31), der(8)t(8;?11) (q24;q11), der(9)

Disease

Papillomavirus-Related Endocervical Adenocarcinoma

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
Viruses: ELISA

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Synonyms

KB 3-1; KB3-1; KB31

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

The KB-3-1 cell line is a well-defined, drug-sensitive subclone isolated from the heterogeneous human cervical epidermoid carcinoma KB cell line (now recognized as a HeLa derivative). It serves as the critical isogenic parental control in one of the most seminal experimental systems for studying the molecular mechanisms of classical multidrug resistance (MDR) in cancer. This cell line was specifically selected for its sensitivity to chemotherapeutic agents, most notably colchicine, and its intrinsic low expression of the MDR1 (ABCB1) gene. Its primary scientific value lies in its direct genetic relationship to a series of progressively resistant sublines (e.g., KB-8-5, KB-C1, KB-V1) that were derived from it through stepwise selection in increasing concentrations of cytotoxic drugs like colchicine or vinblastine.

Identification of Key Biological Processes Associated with Chemoresistance Core Genes

Doxorubicin (DOX) is a widely used chemotherapeutic agent, but its efficacy is often limited by cancer cell resistance. Although multiple DOX resistance mechanisms have been characterized, the global transcriptomic alterations underlying this phenomenon remain poorly understood. The aim of this work was to determine whether a common transcriptional response associated with DOX desensitization exists across tumor cells of different origins and to identify the core elements of this response.

We performed an integrated bioinformatics analysis, including: analysis of independent transcriptomic datasets (comparing DOX-resistant neuroblastoma, breast, and cervical carcinoma cells to their DOX-sensitive counterparts), functional annotation of differentially expressed genes, reconstruction and topology analysis of gene networks, text mining, and survival analysis.

We showed that DOX resistance in cancer cells is associated with cytoskeletal reorganization, modulation of cell adhesion, cholesterol biosynthesis, and dysregulation of mTORC1, Wnt, and Gβγ signaling pathways. Experimental validation in DOX-resistant KB-8-5 cervical carcinoma cells and their sensitive counterparts (KB-3-1) confirmed enhanced cellular adhesion and reduced intracellular cholesterol levels associated with chemoresistance, supporting our in-silico findings.

Identification of key biological processes in KB-3-1 and KB-8-5 cells. — Fig. 1. Phenotypic characterization of KB-3-1 and KB-8-5 cells (Moralev, Arseny D., *et al.*, 2025).

Ask a Question

Write your own review

You May Also Need

CM-1010X

CS-001Y

JHUAS-1

Description: Uterine adenosquamous carcinoma. Said CEA and CA125 producing. Cell growth is slow.

Cat#: CSC-C6608J INQUIRY

View Product

MFE-280

Description: Established from tumor tissue from a 77-year-old woman with recurrence of endometrial carcinoma (adenomatous, partly papillary, grade G3) in 1990; described as forming heterotransplantable tumors in ...

Cat#: CSC-C0454 INQUIRY

View Product