Description
Established from tumor tissue from a 77-year-old woman with recurrence of endometrial carcinoma (adenomatous, partly papillary, grade G3) in 1990; described as forming heterotransplantable tumors in nude mice and as carrying receptors for progesterone
Karyotype
Human hyperdiploid karyotype with 24% polyploidy - 52(48-52)<2n>X, -X, +1, +1, -2, +9, -10, -12, -13, -16, -17, -17, -18, -19, -20, -22, +18mar, add(1)(p34), add(1)(p3?), add(1)(q11), add(3)(q13), add(3)(q27-29), add(4)(p14), der(9)t(9;13)(p11;q11), add(1
Source
endometrium adenocarcinoma
Culture Properties
monolayer
Morphology
epitheloid cells growing adherently in monolayers
Recommended Medium
40% a href="/SuperCult-RPMI-1640-with-L-glutamine-HEPES-CM-1002X-item-2979.htm">RPMI1640+ 40%
EMEM + 20%
h.i. FBS + 2 mM L-glutamine + 1x insulin-transferrin-sodium selenite
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
USA: 1-631-626-9181
Europe: 44-207-097-1828
