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JL-1

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Cat.No.
CSC-C2542
Description
Established from the untreated malignant epithelioid mesothelioma of the pleura of a 54-year-old man with asbestos cancer in 2003
Organism
Human
Karyotype
Human flat-moded karyotype with 12 % polyploidy; 42-47<2n>XY, +5, +7, -10, -13, +20, der(1)t(1;15)(q11;q11), inv(3)(p1?q2?), der(5)t(5;16)(q11;?), add(10)(q25), der(11)t(10;11)(q21;q23), der(14)t(1;14)(q11;q31); sdl with add(9)(p11), del(16)(q11); karyoty
Source
epithelioid mesothelioma
Culture Properties
monolayer
Morphology
adherent, epithelial-like cells growing in monolayers
Recommended Medium
80% DMEM or NCTC-109 + 20% h.i.FBS + 2 mM L-glutamine
Quality Control
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
Viruses: PCR: EBV -,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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