DM-3

Name: DM-3
Brand: Creative Bioarray

Cat.No.: CSC-C2541

Species: Homo sapiens (Human)

Source: Lung

Morphology: adherent, sarcomatoid cells growing in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C2541

Description

Established from a pleural biopsy of a 62-year-old man with malignant mesothelioma

Species

Homo sapiens (Human)

Source

Lung

Recommended Medium

80% DMEM or NCTC-109 + 20% h.i.FBS

Culture Properties

monolayer

Morphology

adherent, sarcomatoid cells growing in monolayers

Karyotype

Human near-diploid karyotype with 16% polyploidy; 46(44-48)<2n>XY; no consistent abnormality detected; resembles published karyotype.

Disease

Pleural Sarcomatoid Mesothelioma

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Synonyms

DM3

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

DM-3 is a human malignant pleural mesothelioma cell line generated from a pleural biopsy of a 62-year-old male patient diagnosed with sarcomatoid mesothelioma. The cell line was initially generated as an in vitro model for exploring the biology aspects of malignant mesothelioma, a very aggressive tumor originating from the mesothelial lining of the pleura. DM-3 cells develop in an adherent pattern and have an elongated spindle-shaped morphology consistent with the sarcomatoid subtype of mesothelioma.

DM-3 has morphological and behavioral characteristics consistent with a more mesenchymal cellular state compared to epithelioid mesothelioma models. Studies with DM-3 have explored cell mobility, cytoskeletal architecture, tumor invasiveness, and treatment responsiveness. DM-3 features a highly elongated fibroblast-like morphology and has been utilized to study the association between cytoskeletal architecture and tumor cell motility in analysis of mesothelioma cell panels. DM-3 has also been used in drug response studies as a model to evaluate susceptibility to anticancer drugs (e.g., proteasome inhibitors, redox-modulating chemicals).

DM-3 has also been included into large-scale cancer cell line collections and molecular profiling efforts, enabling study of mesothelioma-related signaling pathways, genomic changes, and treatment vulnerabilities. These features render DM-3 a suitable experimental model for studies in the biology of sarcomatoid mesothelioma, tumor progression, drug screening and translational oncology.

Heterogeneous Actin Cytoskeleton Organization Across Malignant Mesothelioma Cell Lines

The cytoskeleton is fundamental to tissue homeostasis, controlling cell morphogenesis and migration. Cancer cells show migratory and invasive behaviors that are promoted by dysregulated cytoskeletal dynamics. To study this in MM, Keller et al. analyzed actin filament organization in epithelioid, sarcomatoid and biphasic subtypes of malignant mesothelioma (MM) cell lines.

Cells were seeded on coverslips and after 24 h they were fixed and stained with TRITC-conjugated phalloidin to visualize filamentous actin (F-actin). Epithelioid lines MeT-5A and Mero-25 produced colonies with cortical actin arcs at the periphery, and were tiny and rounded (aspect ratio ~1.6) (Fig. 1A). However, the sarcomatoid line DM-3 showed a fibroblastic morphology with high aspect ratio (3.6) with conspicuous stress fibres throughout the cell (Fig. 1A, C, D). Biphasic lines showed distinct actin organization: JL-1 and STAV-FCS were more elongated (aspect ratios 2.6 and 3.0, respectively) with conspicuous stress fibres whereas STAV-AB had broad lamellipodia at cell borders. Colonies of M-14-K were growing like epithelioid cells while ZL34 had short stress fibres and little condensed membrane ruffles (Fig. 1A). Perinuclear actin caps, short actin filaments capping the nucleus, have been proposed as a structural signature separating epithelial from fibroblast-like cells. Actin caps were extensively established in control BJ fibroblasts, but were not observed in MeT-5A, Mero-25, STAV-AB, and ZL34. For some cells, DM-3, M-14-K, and STAV-FCS had actin caps, while JL-1 retained well-developed caps, similar to fibroblasts.

These results showed the existence of a marked variability in the organization of the actin cytoskeleton in MM cell lines, in agreement with their different morphological and motile features, and suggest the complexity of the cytoskeleton control in the biology of mesothelioma.