Established from a pleural biopsy of a 62-year-old man with malignant mesothelioma
Human near-diploid karyotype with 16% polyploidy; 46(44-48)<2n>XY; no consistent abnormality detected; resembles published karyotype.
adherent, sarcomatoid cells growing in monolayers
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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