Established from a male patient with a well-differentiated primary sigmoid adenocarcinoma of TNM stage T3N0M0
strongly adherent epitheloid cells growing in monolayers and forming islets
Human flat-moded hypotetraploid karyotype with 3.5% polyploidy - 78-85<4n>XX, -Y, -Y, -3, -3, -4, -4, +5, +5, +6, -8, +9, +9, -10, -10, +11, -13, -14, -16, -16, -17, -18, -18, -18, -21, -21, -21, -22, +6-12mar, del(1)(p32)x2, i(5p)x3, add(14)(p11)x2, add(
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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