DMEM/F-12 contains 2.5 mM L-glutamine, 15 mM HEPES, 0.5 mM sodium pyruvate, and 1200 mg/L sodium bicarbonate.
NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.2 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
Sterility tests are performed on each lot of medium using current USP methods. Each lot of medium is also checked for its ability to support the growth of several different cell lines using both sequential subcultures and plating efficiencies. Additional
Storage and Shipping
Store medium at 2 °C to 8 °C in the dark when not in use.
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