Established from Whitlock-Witte type long-term bone marrow culture of BC8 mice
Murine flat-moded hypotetraploid karyotype - 68-77 - centric fusion marker present in most cells
adherent, fibroblast-like cells growing in monolayers
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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