A4-1025

Cat.No.: CSC-C6173X

Species: Mus musculus (Mouse)

Morphology: round single cells in suspension, partly in clusters, lightly adherent

Culture Properties: suspension

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Cat.No.
CSC-C6173X
Description
Described as secreting a mouse monoclonal antibody (IgG2a) detecting all fibers in skeletal muscle and myosin heavy chains on Western blots and detecting mammalian, chicken, zebrafish, axolotl, xenopus skeletal muscle myosin heavy chains; the cell line was established in 1985 by fusion of X63AG8.653 cells with spleen cells from a BALB/c mouse that had been immunized with human skeletal muscle myosin
Species
Mus musculus (Mouse)
Recommended Medium
Culture Properties
suspension
Morphology
round single cells in suspension, partly in clusters, lightly adherent
Karyotype
Murine hypertetraploid karyotype with 5% polyploidy - 84(79-84) - one large submetacentric and up to 5 centric fusion markers present
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: secretion of a IgG2a monoclonal antibody
Viruses: ELISA: reverse transcriptase positive
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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