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A4-1025
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Cat.No.
CSC-C6173X
Description
Described as secreting a mouse monoclonal antibody (IgG2a) detecting all fibers in skeletal muscle and myosin heavy chains on Western blots and detecting mammalian, chicken, zebrafish, axolotl, xenopus skeletal muscle myosin heavy chains; the cell line was established in 1985 by fusion of X63AG8.653 cells with spleen cells from a BALB/c mouse that had been immunized with human skeletal muscle myosin
Species
Mouse
Source
hybridoma (anti-fiber/myosin)
Recommended Medium
Culture Properties
suspension
Morphology
round single cells in suspension, partly in clusters, lightly adherent
Karyotype
Murine hypertetraploid karyotype with 5% polyploidy - 84(79-84) - one large submetacentric and up to 5 centric fusion markers present
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: secretion of a IgG2a monoclonal antibody
Viruses: ELISA: reverse transcriptase positive
Immunology: secretion of a IgG2a monoclonal antibody
Viruses: ELISA: reverse transcriptase positive
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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