Established from a Rauscher murine leukemia virus-induced erythroid leukemia of a female DBA/2 mouse; cells were described to be responsive to erythropoietin (EPO) and to form benzidine-positive colonies upon stimulation with EPO or DMSO
Murine hypodiploid karyotype with 10% polyploidy - 39(38-40), t(X;1)(q10;q10) - two centric-fusion markers present - resembles published karyotype
round, single cells growing in suspension
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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