Species: mouse - male, 100 days old; Mus musculus
Note: this grows in chemically defined medium; the cells attach very lightly to the substrate, and as the culture becomes heavy some of the cells will detach into the medium; tested and found negative for ectromelia virus (mousepox)
connective tissue; subcutaneous; areolar and adipose
100% NCTC 135 medium
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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