KYSE-270

Cat.No.: CSC-C0428

Species: Homo sapiens (Human)

Source: Esophagus

Morphology: epitheloid cells growing in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0428

Description

Derived from well differentiated invasive esophageal squamous cell carcinoma resected from middle intra-thoracic esophagus of a 79-year-old Japanese man prior to treatment (depth of invasion was not beyond the muscularis propria); described to carry p53 mutation

Species

Homo sapiens (Human)

Source

Esophagus

Recommended Medium

49%RPMI1640 + 49%Ham's F-12 + 2%h.i. FBS.

Culture Properties

monolayer

Morphology

epitheloid cells growing in monolayers

Karyotype

Human hypotriploid karyotype with 16% polyploidy - 66(64-67)<3n>XX/XXX, +1, -2, +3, -5, -6, +7, +8, -9, -10, -15, -15, -17, -18, -19, -20, -22, +5mar (1bisat), ?del(X)(q25), add(1)(p1?), add(1)(p2?), i(1p), der(1)t(1;?)(?;2)(q13;?)(?;q11), add(2)(q37), de

Disease

Esophageal Squamous Cell Carcinoma

Quality Control

Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 - , cytokeratin-8 +, cytokeratin-17 (+), cytokeratin-18 +,

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Synonyms

KYSE 270; KYSE270; Kyse270; KY-270

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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