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FDCP-1
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Cat.No.
CSC-C2761
Description
Established from the long-term bone marrow culture of B6D2F1 mice; cells are IL-3-dependent; their differentiation is blocked; this subclone is responsive to G-CSF (FDCP means "factor dependent cell-Paterson" after the Paterson Insitute, Manchester, UK)
Species
Mouse
Source
bone marrow
Recommended Medium
70% SuperCult® IMDM + 20%horse serum + 10% vol conditioned medium of WEHI-3B cell line
Culture Properties
suspension
Morphology
single or clustered cells growing in suspension
Karyotype
Murine hypodiploid karyotype - 32<2n> - carries 8 centric fusion markers
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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