Established from the long-term bone marrow culture of B6D2F1 mice; cells are IL-3-dependent; their differentiation is blocked; this subclone is responsive to G-CSF (FDCP means "factor dependent cell-Paterson" after the Paterson Insitute, Manchester, UK)
Murine hypodiploid karyotype - 32<2n> - carries 8 centric fusion markers
single or clustered cells growing in suspension
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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