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DMBM-2
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Cat.No.
CSC-C1085
Description
Established from the bone marrow of a C3H/HeJ mouse
Species
Mouse
Source
macrophages
Recommended Medium
80%RPMI-1640 + 1 mM sodium pyruvate + 1 mM non-essential amino acids + 20% horse serum + 1.25 μg/ml vitamin B12 + 75 μM alpha-thioglycerin + 2 mM L-glutamine + 2.5% 10x L-929 supernatant
Morphology
round cells growing in aggregates, mostly loosely adherent, some cells have long processes
Karyotype
Murine near-diploid karyotype with 10% tetraploidy - 42(37-43)<2n> - centric fusion markers absent
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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