Established from the bone marrow of a C3H/HeJ mouse
Murine near-diploid karyotype with 10% tetraploidy - 42(37-43)<2n> - centric fusion markers absent
round cells growing in aggregates, mostly loosely adherent, some cells have long processes
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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