DMBM-2

Cat.No.: CSC-C1085

Species: Mus musculus (Mouse)

Source: Bone Marrow

Morphology: round cells growing in aggregates, mostly loosely adherent, some cells have long processes

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Cat.No.

CSC-C1085

Description

Established from the bone marrow of a C3H/HeJ mouse

Species

Mus musculus (Mouse)

Source

Bone Marrow

Recommended Medium

80%RPMI-1640 + 1 mM sodium pyruvate + 1 mM non-essential amino acids + 20% horse serum + 1.25 μg/ml vitamin B12 + 75 μM alpha-thioglycerin + 2 mM L-glutamine + 2.5% 10x L-929 supernatant

Morphology

round cells growing in aggregates, mostly loosely adherent, some cells have long processes

Karyotype

Murine near-diploid karyotype with 10% tetraploidy - 42(37-43)<2n> - centric fusion markers absent

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

DMBM-2 is a murine macrophage cell line derived from the bone marrow of a C3H/HeJ animal. The cell line was established by spontaneous transformation during long-term culture and frequently utilized as an in vitro model for studies on macrophage biology and innate immune responses. DMBM-2 cells have the typical macrophage morphology, characterized by round to irregular-shaped cells with mostly loose aggregation and partial adherence. Some cells exhibit lengthy cytoplasmic processes, which are indicative of their activated macrophage-like nature.

DMBM-2, as a model of bone marrow-derived macrophages, preserves many of the functional traits associated with professional phagocytes, such as response to inflammatory stimuli, generation of cytokines, and engagement in host defence mechanisms. The parental C3H/HeJ mouse strain is known for increased lipopolysaccharide (LPS) responsiveness and has been used in research of macrophage activation pathways, innate immunological signaling and mechanisms of inflammatory control, and DMBM-2 has been used in these studies.

DMBM-2 cells offer a straightforward and reproducible alternative to primary cultures of macrophages for the assessment of cytokine responses, cell-pathogen interactions and immune-modulating chemicals. They have also been employed to investigate signal transmission, macrophage differentiation, phagocytic activity and generation of inflammatory mediators. DMBM-2 cells, with their consistent growth properties and well-defined macrophage phenotype, are suitable for experimental studies in immunology, inflammation and host-pathogen interaction.

Species- and Cell-Type-Specific Lysosomal Characteristics in Macrophage Models of Phospholipidosis

Phospholipidosis (PLD), the intracellular accumulation of phospholipids, is a key toxicological response to cationic amphiphilic drugs (CADs) and can impair alveolar macrophage function. To optimize PLD screening, Öhlinger et al. evaluated a panel of lysosomal stains (LysoSensor, Acridine Orange, Nile Red, HCS LipidTOX, LysoID) across murine (DMBM-2, J774, RAW264.7) and human macrophage models (THP-1, monocyte-derived M1/M2 macrophages).

Macrophages grouped into four size categories: DMBM-2/human M1 (largest), THP-1, J774/human M2, and RAW264.7 (smallest) (Fig. 1). Lysosome quantification revealed distinct interspecies differences: murine cells had higher lysosome density (0.11-0.13/µm²) than human cells (0.05-0.06/µm²). Human macrophages possessed fewer but larger lysosomes, whereas murine macrophages had smaller, more numerous lysosomes. Correlation analysis showed strong linkage between lysosome area and dye intensity in J774 and THP-1 (r = 0.63-0.68), but weaker correlation in DMBM-2 and RAW264.7 (r = 0.42-0.48). Post-CAD exposure, cell densities varied regionally (60-134% of control) with no dose-dependency, suggesting spatial heterogeneity rather than cytotoxicity (Fig. 2). These findings highlight pronounced species-specific differences in macrophage lysosomal architecture that must be considered when selecting models for PLD assessment.

Cell area of macrophages when exposed to the solvent controls. Means ± SEM are shown and groups indicated with letters. — Fig. 1. Cell area of macrophages when exposed to the solvent controls. Means ± SEM are shown and groups indicated with letters (Öhlinger K, Absenger-Novak M, *et al.*, 2020).

Dose-dependent changes in cell density. — Fig. 2. Dose-dependent changes in cell density (Öhlinger K, Absenger-Novak M, *et al.*, 2020).

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