Cells are a subclone derived from B13.29 (first described as clone 9.9) by fusion of BALB/c spleen cells with SP2/0-AG14 myeloma cells; this hybridoma is a non-producer. B9 is a mouse B cell hybridoma line that requires Interleukin 6 (IL-6) for survival and proliferation. IL-6 is a pluripotent cytokine with multiple effects on many different cell types. It is produced by a variety of cells in response to various stimuli.
Apparently murine chromosome morphology with extensive centric fusions
round cells growing in suspension
+ 50µM 2-Mercaptoethanol (2ME) + 50pg/ml recombinant human IL-6 + 5% h.i.FBS
. IL-6 needs to be added fresh at each media change.
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
liquid nitrogen vapor phase
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