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B9

  • Specification
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Cat.No.
CSC-C6191X
Description
Cells are a subclone derived from B13.29 (first described as clone 9.9) by fusion of BALB/c spleen cells with SP2/0-AG14 myeloma cells; this hybridoma is a non-producer. B9 is a mouse B cell hybridoma line that requires Interleukin 6 (IL-6) for survival and proliferation. IL-6 is a pluripotent cytokine with multiple effects on many different cell types. It is produced by a variety of cells in response to various stimuli.
Organism
Mouse
Karyotype
Apparently murine chromosome morphology with extensive centric fusions
Source
Spleen
Culture Properties
Suspension
Morphology
round cells growing in suspension
Recommended Medium
DMEM or RPMI-1640 + 50µM 2-Mercaptoethanol (2ME) + 50pg/ml recombinant human IL-6 + 5% h.i.FBS. IL-6 needs to be added fresh at each media change.
Quality Control
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
liquid nitrogen vapor phase
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6256W
CSC-6264W
CSC-6284W
CSC-6285W
CSC-6286W
CSC-6287W
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