B9

Specification
Recommended Products

Cat.No.

CSC-C6191X

Description

Cells are a subclone derived from B13.29 (first described as clone 9.9) by fusion of BALB/c spleen cells with SP2/0-AG14 myeloma cells; this hybridoma is a non-producer. B9 is a mouse B cell hybridoma line that requires Interleukin 6 (IL-6) for survival and proliferation. IL-6 is a pluripotent cytokine with multiple effects on many different cell types. It is produced by a variety of cells in response to various stimuli.

Species

Mouse

Source

Spleen

Recommended Medium

DMEM or RPMI-1640 + 50µM 2-Mercaptoethanol (2ME) + 50pg/ml recombinant human IL-6 + 5% h.i.FBS. IL-6 needs to be added fresh at each media change.

Culture Properties

Suspension

Morphology

round cells growing in suspension

Karyotype

Apparently murine chromosome morphology with extensive centric fusions

Quality Control

Tests for mycoplasma, bacteria and fungi were negative

Storage and Shipping

liquid nitrogen vapor phase

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

CM-1002X

SuperCult® RPMI-1640 with L-glutamine & HEPES

CM-1010X

CS-001Y

For research use only. Not for any other purpose.

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