Immortalized Equine Kidney Cells (extEqFK)
Cat.No.: CSC-I9292L
Species: Equine
Source: Kidney
Morphology: Polygonal
Culture Properties: Adherent
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Note: Never can cells be kept at -20 °C.
2) PCR analysis for HPV16 E6/E7 presence
Immortalized Equine Kidney Cells (extEqFK) are an established cell line from the kidney of the horse (Equus caballus) originally created for use in research to allow for long-term and renewable cultures of equine kidney cells in vitro. Immortalization of extEqFK cells was achieved by transduction with human papillomavirus 16 (HPV16) E6/E7 oncogenes through non-replicating retroviral transduction (PA317 LXSN HPV16 E6/E7).
The extEqFK cell line is adherent with epithelial like morphology growing as a monolayer in vitro, like their tissue of origin. These cells are commonly used to model renal cell biology, host-pathogen interactions, and equine-specific cellular responses. Applications of extEqFK primarily include their use in veterinary virology research where they are commonly utilized to study equine infections. ExtEqFK cells have also been used in toxicology/pharmacology assays as well as research into kidney-specific cell biology from the horse. Immortalized Equine Kidney cells can be passaged indefinitely in culture, overcoming the problems of primary cells while maintaining phenotypic and functional consistency.
Isolation of Equid Alphaherpesvirus 3 from a Horse in Iceland with Equine Coital Exanthema
Equine coital exanthema (ECE) caused by equid alphaherpesvirus 3 (EHV-3) is a contagious venereal disease. Three types of equine herpesviruses (EHV) have been found in Iceland, EHV-4, EHV-2 and EHV-5, while EHV-1 has never been detected. Thorsteinsdóttir et al. isolated EHV-3 from a mare with papules on the vulva and inoculated in equine kidney cells (Fig. 1). It has been shown that EHV-3 replicates almost exclusively in cells of equine origin. They therefore tested the growth of dilutions of the EHV-3 virus stock in five different cells culture systems and determined the cytopathy with a light microscope. Three different equine fetal cells were tested, both primary cells and cells with extended life span. The primary cells can be passage about 10-12 times as compared to 40 passages for the cells with extended life span. The titers were 105.7, 105.11 and 105.56 TCID50/mL, respectively, for the three equine cell culture tested, prmEqFK, extEqFK and extEqFL. As expected, no CPE was observed in the RK13 and Vero cells as they are not equine cells.
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