Mouse Embryonic Stem Cells (MSCs) are isolated from day 3.5 (E3.5) blastocyts of pathogen-free laboratory mice. The established ES cell lines are propagated by co-culture with mouse embryonic fibroblasts (MEF) as described in standard mouse ESC culture protocol. Mouse Embryonic Stem Cell-Derived Endothelial Cells (ES-ECs) from Creative Bioarray are isolated from MESCs. ES-EPCs are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Creative Bioarray's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen.
ES-ECs can be used in assays of cell to cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request.
Never can primary cells be kept at -20 °C.
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