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C57BL/6 Mouse Primary Lung Fibroblasts

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Cat.No.
CSC-C1880
Description
C57BL/6 Mouse Primary Lung Fibroblasts from Creative Bioarray are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Lung Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 5x10^5 cells per ml and is delivered frozen.
Species
Mouse: C57BL/6
Organism
Mouse
Source
Lung
Morphology
Multipolar
Recommended Medium
SuperCult® Murine Fibroblast Medium
Quality Control
Mouse Primary Lung Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended. Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Never can primary cells be kept at -20 °C
Cell Type
Fibroblasts
Application
Pathogenesis of pulmonary fibrosis;
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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CONTACT US USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: 

Europe
Tel: 44-207-097-1828
Email: