Immortalized Human Renal Proximal Tubule Cells-hTERT
Cat.No.: CSC-I0721Z
Species: homo sapiens
Source: Kidney
Cell Type: Epithelial Cells
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Cat.No.
CSC-I0721Z
Description
Immortalized Human Renal Proximal Tubule Cells-hTERT have been obtained immortalizing Human Renal Proximal Tubule Cells with Lenti-hTERT Lentivirus. Immortalized cells were controlled passaging side by side with the primary cells. Primary cells go into senescence after the 8th passage while the hTERT transduced cells go beyond 30 passages. This cell line is a great in vitro model system.
Species
                                homo sapiens
                            Source
                                Kidney
                            Recommended Medium
                                SuperCult® Immortalized Human Renal Proximal Tubule Cell Medium (Cat No.: CM-I2072Z)
                            Freezing Medium
                                Complete medium supplemented with 10% (v/v) DMSO
                            Immortalization Method
                                Human telomerase reverse transcriptase (hTERT)
                            Application
                                For Research Use Only
                            Cell Type
                                Epithelial Cells
                            Growth Properties
                                Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
                            Quality Control
                                Real Time PCR was used to quantify hTERT gene expression in immortalized cell line.
free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations
                            free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations
Storage and Shipping
                                Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
                            Citation Guidance
                        
                            If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
                            
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