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Immortalized Rat Retinal Precursor Cells (R28)
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Cat.No.
CSC-I9196L
Description
The Immortalized Rat Retinal Precursor Cell Line (R28) is derived from postnatal day 6 Sprague-Dawley rat retinal tissue immortalized with the 12S E1A gene of adenovirus. Studies have shown that this cell line possesses retinal neurotransmitter receptors that can respond to neurotransmitter stimulation (i.e. dopamine, serotonin, glycine and acetylcholine) and subpopulations are immunoreactive to GluR1, 2 and 3, N-methyl-D-aspartate (NMDA), and γ-aminobutyric acid-a (GABAa). In addition, neurite growth can be promoted by varying the culture medium condition.The heterogeneity of this Immortalized Rat Retinal Precursor Cell Line provides a diversity of cell types in which more closely simulate a retinal explant and offers differentiation potentials useful in studies involving retinal function. Recently amicroarray datasetof R28 cells is published describing the presence and absence of 8799 genes and ESTs that may relevant for investigators with an interest in using this cell line for a particular molecular analysis.
Source
Retinal Tissue
Culture Properties
Adherent
Immortalization Method
Serial passaging and transduction with retroviruses carrying 12S E1A gene
Markers
IRBP, S-antigen
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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Quality Control
1) Western blotting and immunocytochemistry were used to confirm the E1A transgene expression
2) RT-PCR and immunocytochemistry were used to assess the expression of the photoreceptor specific marker S-antigen and interphotoreceptor retinoid binding protein (IRBP)
2) RT-PCR and immunocytochemistry were used to assess the expression of the photoreceptor specific marker S-antigen and interphotoreceptor retinoid binding protein (IRBP)
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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