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Immortalized Rat Retinal Müller Cells (rMC-1)
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Cat.No.
CSC-I9199L
Description
Müller cells are glial cells in the vertebrate retina responsible for the homeostatic and metabolic support of retinal neurons. Under pathological conditions, a subset of Müller cells may differentiate to neural progenitor/stem cells which regenerate lost photoreceptors and neurons.The rMC-1 cell line is derived from stable transformation of SV40 antigen into primary rat retinal Müller cells. The rMC-1 cells resembles its primary counterpart and expresses reactive gliosis marker GFAP and Müller cell-specific marker CRALBP. This cell line enables mammalian retinal function research, as well as facilitates gene expression studies in Müller cells-neuron and/or Müller cells-endothelial cell interactions.
Source
Retina
Culture Properties
Adherent
Morphology
Large, flat cells
Immortalization Method
Serial passaging and Calcium Phosphate mediated transfection with simian virus 40 (SV40) DNA
Markers
GFAP, CRALBP
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1) Immunocytochemistry and immunoblotting were used to detect the presence of glial fibrillary acidic protein (GFAP) and cellular retinaldehyde-binding protein (CRALBP) ;
2) Immunocytochemistry was used to confirm the nuclear expression of SV40-LTA in the immortalized cells.
2) Immunocytochemistry was used to confirm the nuclear expression of SV40-LTA in the immortalized cells.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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