Recombinant Human Interleukin-1 beta (rHu IL-1 beta) is a 17.0 kDa protein containing 153 amino acid residues. rHu IL-1 beta is a potent immuno-modulator, which mediates a wide range of immune and inflammatory responses including the activation of B- and T-cells.
Human IL1B expressed in E.coli
CAT# CSC-CTK0212-10 (10 μg); CAT# CSC-CTK0212-50 (50 μg)
Greater than 98% as determined by SDS-PAGE and RP-HPLC analysis.
The specific biological activity of 2 x 10^8 IU/mg was determined in the test of augmentation of lymphocyte proliferation assay using mouse thymus.
Less than 1 EU/μg.
Lyophilized from a sterile-filtered, concentrated protein solution (1 mg/ml) containing 50 mM phosphate buffer (pH 7.1) and 150 mM NaCl..
Please centrifuge product briefyly before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water or PBS to a concentration of 0.1-1.0 mg/ml. For further dilution into other aqueous buffers carrier protein ( 1%) should be added to avoid loss of bioactivity. This solution can then be diluted and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-1 beta should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze/thaw cycles.
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