KYSE-30

Cat.No.: CSC-C0405

Species: Homo sapiens (Human)

Source: Esophagus

Morphology: adherent, epitheloid with long processes growing in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0405

Description

Derived from well differentiated invasive esophageal squamous cell carcinoma resected from middle intra-thoracic esophagus of a 64-year-old Japanese man prior to treatment; cell line established from tumor cells heterotransplanted into athymic mice; described to be heterotransplantable in athymic mice and to carry p53 mutation and amplification of cERB B, MYC and CYCLIN D1

Species

Homo sapiens (Human)

Source

Esophagus

Recommended Medium

45%RPMI1640 + 45%Ham's F-12 + 10%h.i. FBS.

Culture Properties

monolayer

Morphology

adherent, epitheloid with long processes growing in monolayers

Karyotype

Highly rearranged hypertriploid karyotype with 8% polyploidy - 66-74<3n>Xder(X), -Y, +1, +1, -5, +7, -9, -10, -15, +16, -18, -18, -19, -21, -21, -21, -22, -22, +12-15mar, t(X;2)(p11;p11), ins(1;1)(p34/36;q21q32), add(1)(q11), der(1)add(1) (p21)(q22), del(

Disease

Esophageal Squamous Cell Carcinoma

Quality Control

Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cy

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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