KYSE-30

Specification
Recommended Products
Publications

Cat.No.

CSC-C0405

Description

Derived from well differentiated invasive esophageal squamous cell carcinoma resected from middle intra-thoracic esophagus of a 64-year-old Japanese man prior to treatment; cell line established from tumor cells heterotransplanted into athymic mice; described to be heterotransplantable in athymic mice and to carry p53 mutation and amplification of cERB B, MYC and CYCLIN D1

Species

Human

Source

esophageal squamous cell carcinoma

Recommended Medium

45%RPMI1640 + 45%Ham's F-12 + 10%h.i. FBS.

Culture Properties

monolayer

Morphology

adherent, epitheloid with long processes growing in monolayers

Karyotype

Highly rearranged hypertriploid karyotype with 8% polyploidy - 66-74<3n>Xder(X), -Y, +1, +1, -5, +7, -9, -10, -15, +16, -18, -18, -19, -21, -21, -21, -22, -22, +12-15mar, t(X;2)(p11;p11), ins(1;1)(p34/36;q21q32), add(1)(q11), der(1)add(1) (p21)(q22), del(

Quality Control

Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cy

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

CM-1002X

SuperCult® RPMI-1640 with L-glutamine & HEPES

INQUIRY

CM-1449Y

SuperCult® Ham's F-12 Nutrient Mixture

INQUIRY

CS-001Y

PremSera® FBS

INQUIRY

For research use only. Not for any other purpose.

Q & A
Customer Review

How to judge the quality of serum by its appearance?

If the serum is cloudy and opaque, it means that the serum is contaminated or the protein in the serum is denatured; if the serum is brownish red, it means that the hemoglobin content in the serum is too high and there is hemolysis when the material is taken; if the liquid feels thin when shaking, it means that the serum is mixed with too much saline.

Ask a Question

Average Rating: 5.0 | 1 Scientist has reviewed this product

A wide range of products

Creative Bioarray has a complete range of cell products and services, bringing a lot of convenience to our experiments.

19 Jan 2023

Ease of use
After sales services
Value for money

Write your own review

Customer Support & Price Inquiry