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Porcine Dermal Fibroblasts- Neonatal
- Specification
- Recommended Products
Cat.No.
CSC-C4889L
Description
Porcine Primary Dermal Fibroblasts are isolated from skin tissue of porcine. Porcine Primary Dermal Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and is delivered frozen.
Source
Dermis
Recommended Medium
Cell Type
Fibroblast
Disease
Normal
Quality Control
Porcine Primary Dermal Fibroblasts are tested for negative expression of von Willebrand Factor Expression/Factor VIII, cytokeratin 18, and alpha smooth muscle actin. Porcine Primary Dermal Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.
Never can cryopreserved cells be kept at -20 °C.
Never can cryopreserved cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Q:
Is it possible to increase the serum ratio if the cells are slow growing?
A:
That's certainly possible!
Average Rating: 4.0 | 1 Scientist has reviewed this product
Unique
Cell products had no problems from resuscitation, subculture to freezing and storage.
12 Aug 2023
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