Porcine Brain Vascular Fibroblasts

Specification
Recommended Products

Cat.No.

CSC-C4885L

Description

Porcine Brain Vascular Fibroblasts from Creative Bioarray are isolated from brain tissue of porcine. Porcine Brain Vascular Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution and incubated in Creative Bioarray Complete Growth Medium. The cells are shipped at passage 2. Each vial contains at least 0.5x10^6 cells per ml and is delivered frozen.

Species

Porcine

Source

Brain

Recommended Medium

SuperCult® Porcine Brain Vascular Fibroblast Medium

Cell Type

Fibroblast

Disease

Normal

Quality Control

Porcine Primary Brain Vascular Fibroblasts are tested for negative expression of von Willebrand Factor Expression/Factor VIII, cytokeratin 18, and alpha smooth muscle actin. Porcine Primary Brain Vascular Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.

Storage and Shipping

Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.
Never can cryopreserved cells be kept at -20 °C.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.

Q & A
Customer Review

How to determine fibroblasts in primary cells?

Only after 3 or more generations of passaging culture can we obtain relatively pure fibroblasts. Polygonal cells can be seen around the tissue block at 7-8 d of culture and grow in patches all around, and the cells are passaged at 13 d of culture. The cells obtained by digestion method were cultured for 5 d, and the cells started to grow rapidly around them and were ready for passaging at about 11-12 d. After passaging, the cells grew all over the culture plate in 6 d.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

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We have always relied on this product because it has helped us to complete our experiments well.

17 July 2022

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