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Human Hypertension Pulmonary Artery Fibroblasts

  • Specification
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Cat.No.
CSC-C5001J
Description
Human hypertension pulmonary artery fibroblasts are isolated from the carotid artery of human donor that has been diagnosed with hypertension. Human hypertension pulmonary artery fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in complete growth medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml.
Species
Human
Source
Lung; Artery
Cell Type
Fibroblast
Disease
Diseased
Quality Control
Human hypertension pulmonary artery fibroblasts are negative for bacteria, yeast, fungi and mycoplasma.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.
Never can cryopreserved cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

  • Q & A
  • Customer Review
Q:
Is there a limit to the number of stem cell passages?
A:

Theoretically, there is no limit to the number of passages, but in practice, due to changes in the culture environment of the isolated culture, serum quality, medium quality, passaging damage and other external culture environment, the cells will have problems of differentiation, non-proliferation, aging and apoptosis if they are more than 10 generations old, so it is recommended to use them in less than 10 generations in regular practice.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

Multiple purchases

I bought Creative Bioarray's cellular products on the recommendation of a friend, and I have returned many times since then.

13 Mar 2022


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