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Human Hypertension Pulmonary Vein Smooth Muscle Cells
- Specification
- Recommended Products
Cat.No.
CSC-C5008J
Description
Human hypertension pulmonary vein smooth muscle cells are isolated from the pulmonary vein of human donor that has been diagnosed with hypertension. Human hypertension pulmonary vein smooth muscle cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in complete growth medium generally for 3-7 days. Prior to shipping, cells at passage 1 are detached from the culture flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and is delivered frozen.
Species
Human
Source
Lung; Vein
Recommended Medium
Cell Type
Smooth Muscle
Disease
Diseased
Quality Control
Human hypertension pulmonary vein smooth muscle cells are negative for bacteria, yeast, fungi and mycoplasma.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.
Never can cryopreserved cells be kept at -20 °C.
Never can cryopreserved cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Q:
What if the primary cells have more red blood cells? Do I need erythrocyte lysate?
A:
If there are more red blood cells in the early stage of isolation, they can be separated with Lymphocyte Separation Solution or Percoll Separation Solution, or treated with Lysis Solution.
Average Rating: 5.0 | 1 Scientist has reviewed this product
Patient and Professional
The staffs were very patient and offered many solutions to the cell contamination problems.
17 Mar 2021
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