BALB/c mouse bone marrow dendritic cells are derived from the tibias and femurs of pathogen-free laboratory adult mice. Cells are grown in 100 mm treated tissue culture dish and incubated in complete growth medium for 5-7 days. Prior to shipping, cells are detached from dishes and immediately cryo-preserved in vials. Each vial contains at least 5 x10^6 cells and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of markers using antibodies, CD11c by flow cytometry. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Creative Bioarray.
BALB/c mouse bone marrow dendritic cells are negative for bacteria, yeast, fungi and mycoplasma.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.
Never can cryopreserved cells be kept at -20 °C.
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