Primary human bone marrow hematopoietic progenitors are multipotent cells that can differentiate into several lineages (e.g., myeloid, erythroid and megakaryocytic cells). The Erythroid SuperCult® Supplement allow differentiation of CD34+ cells into the erythroid lineage in 96-well plates.
10 Day Simple Protocol: Simply add appropriate volume of media (Lymphocyte Growth Medium) to dilute supplement and mix with CD34+ cells in media. Plate into 96-well plate; differentiation complete in 10 days.
• SuperCult® Supplement A - Erythroid: Sufficient supplement for 1 x complete 96-well plate
• SuperCult® Supplement B - Erythroid: Sufficient supplement for 3 x complete 96-well plates
Features & Benefits:
• No need for methylcellulose or any substrate
• No manual counting
• Differentiation via quantification of the expression of lineage-specific markers
SuperCult® Supplement - Erythroid Supplement are tested to ensure erythroid differentiation of bone marrow CD34+ cells by examination of Erythroid Glycophorin A expression. SuperCult® Supplement - Erythroid Supplement tests negative for bacteria, yeast, and fungi.
Recommended Cells: Bone Marrow CD34+ Cells
Recommended Medium: Lymphocyte Growth Medium
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