The Melanocyte Medium (MelM) is a complete medium designed for optimal growth of normal human dermal-derived melanocytes in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and low percent of fetal bovine serum (0.5%). It is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human melanocytes in vitro.
MelM consists of 500 ml of basal medium, 5 ml of melanocyte growth supplement, 2.5 ml of fetal bovine serum and 5 ml of penicillin/streptomycin solution.
Storage and Shipping
Store the basal medium at 4 °C, the MelGS, FBS and the P/S solution at -20 °C. Protect from light. Gel ice.
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