MCDB 131 Medium provides a defined and nutritionally balanced environment for mammalian cell culture. The medium includes trace elements and higher levels of certain vitamins and amino acids compared to basal medium. MCDB 131 Medium was originally developed for human microvascular endothelial cell (HUVEC) culture, but supports the growth of other cell types, including hepatocytes, smooth muscle cells, and cardiac myocytes.
Supplement with glutamine prior to use. For vascular endothelial cells, 10 mM glutamine (final concentration) is recommended. For other cell lines, 2 mM glutamine (final concentration) is generally required; however, the optimal concentration is dependent on your cell line of interest.
Sterility tests are performed on each lot of medium using current USP methods. Each lot of medium is also tested for the ability to support the growth of several different cell lines using both sequential subcultures and plating efficiencies.
Storage and Shipping
Store medium at 2 °C to 8 °C in the dark when not in use.
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