Fibroblast Medium-animal component free (FM-acf) is a complete medium designed for optimal growth of normal human fibroblasts in vitro. It is a sterile, liquid medium which contains non-animal source of essential and non-essential amino acids, vitamins, lipids, organic and inorganic compounds, hormones, growth factor, trace minerals and plant extract. The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human fibroblasts in vitro.
Storage and Shipping
Store the basal medium at 4 °C, the FGS-acf and the P/S solution at -20 °C. Protect from light. Gel ice.
FM-acf consists of 500 ml of basal medium, 5 ml of fibroblast growth supplement-animal component free, and 5 ml of penicillin/streptomycin solution.
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