Custom-Made Endothelial Cell Medium is a medium designed for the culture of endothelial cells. It was tested and optimized with mouse or human endothelial cell growth and proliferation in vitro. It is formulated for use with 5% CO2 and 95% air in a humidified incubator.
The medium consists of 500 ml of basal medium (containing essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals), supplemented with endothelial cell growth supplement, and antibiotics.
Serum-Free Endothelial Cell Medium Supplement Kit includes:
0.5 ML VEGF
0.5 ML EGF
0.5 ML ECGS
0.5 ML Heparin
0.5 ML Hydrocortisone
5.0 ML L-Glutamine
5.0 ML Antibiotic-Antimycotic Solution
25.0 ML FBS
Storage and Shipping
Store the basal medium at 2-8 °C. Store endothelial cell growth supplement, fetal bovine serum (FBS) and antibiotics at -20 °C. The complete cell culture medium with Supplement Kit can be kept in 4 °C for two months. Protect from light.
To assure sterility, the prepared medium should be filtered with a 0.2 um filter after 2 weeks or if there is concern that sterility was compromised during the supplementation process.
Ice pack and dry ice.
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