ActoFactor™ Recombinant Human Transforming growth factor, alpha
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Cat.No.
CSC-CTK0332
Description
Recombinant Human Transforming Growth Factor-alpha (rHu TGF-alpha) is a polypeptide growth factor, which stimulates the proliferation of a wide range of epidermal and epithelial cells. RHu TGF-alpha has been shown to induce transformation anchorage independence in cultured cells. Human TGF-alpha is a 5.5 kDa protein containing 50 amino acid residues.
Species
Human
Product Overview
Human TGFA expressed in E.coli
Molecular Mass
5.5 kDa
Size
CAT# CSC-CTK0332-100 (100 μg); CAT# CSC-CTK0332-500 (500 μg)
Expression System
E.coli
Purity
Greater than 98% as determined by SDS-PAGE and HPLC analysis.
Activity
The ED50 as determined by the dose-dependent stimulation of the proliferation of BALB/c 3T3 cells is ≤ 0.2 ng/ml, corresponding to a specific activity of Greater than 5 x 10^6 units/mg.
Endotoxin Level
Less than 1 EU/μg.
Formulation
The sterile filtered solution was lyophilized with no additives.
Reconstitution
Please centrifuge product before opening the vial. The lyophilized rHu TGF-alpha should be reconstituted in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized rHu TGF-alpha, though stable at room temperature, is best stored desiccated at - 20°C. Reconstituted rHu TGF-alpha should be stored in working aliquots at -20°C. Avoid repeated freeze / thaw cycles.
Citation Guidance
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