ActoFactor™ Recombinant Human Erythropoietin (CHO)

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ActoFactor™ Recombinant Human Erythropoietin (CHO)

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Cat.No.
CSC-CTK0098
Description
Recombinant Human Erythropoietin-alpha, produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology, is a single polypeptide chain of 165 amino acids, a predicted molecular mass of 21,000 Dalton and an apparent glycosylated molecular mass of 36-40 kDa.
Organism
Human
Product Overview
Human EPO expressed in CHO cells
Molecular Mass
21 kDa
Size
CAT# CSC-CTK0098-50 (50 μg); CAT# CSC-CTK0098-250 (250 μg)
Expression System
CHO cells
Purity
Greater than 98% as determined by SDS-PAGE and HPLC analysis.
Activity
Erythropoietin-alpha is fully biologically active when compared to a standard. The specific activity was measured by Normocyth-aemic mice and was found to be 150,000 IU/mg.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Sterile-filtered, white, lyophilized (freeze-dried)powder. Recombinant Erythropoietin-alpha was lyophilized after extensive dialysis against a buffer containing 0.59 mg sodium citrate, 0.58 mg sodium chloride and 0.006 mg citric acid per liter.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu EPO-alpha should be used immediately or stored long- term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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