Fibroblast growth factor-9 (FGF-9) is a steroid-regulated mitogen and survival factor for nerve and mesenchymal cells. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-9 targets glial cells, astrocytes cells and other cells that express the FGFR 1c, 2c, 3b, 3c, and 4. Rat and mouse FGF-9 show a very high homology to human FGF-9. The transcripts for FGF-9 have been found in brain and in kidney tissue. Fibroblast Growth Factor-9 is a member of the fibroblast growth factor (FGF) family. Recombinant rat FGF-9 produced in E. coli is a single, non-glycosylated polypeptide chain of 205 amino acids and a molecular mass of 23,308 Dalton. It has been purified by proprietary chromatographic techniques.
Rat Fgf9 expressed in E.coli
CAT# CSC-CTK0806-10 (10 μg); CAT# CSC-CTK0806-50 (50 μg)
Greater than 95% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.
Recombinant murine FGF-9 is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake), is <0.5 ng/ml corresponding to a specific activity of 2 x 10^6 Units/mg.
Less than 1 EU/μg.
Lyophilized (freeze-dried), white powder. The prote in was lyophilized from a concentrated (1 mg/ml), sterile-filtered protein solution in 10 mM phosphate buffer (pH 7.5) and 75 mM ammonium sulfate.
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rRa FGF-9 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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