Recombinant rat MIP-1 alpha produced in E. coli is a single, non-glycosylated polypeptide chain of 69 amino acids and a molecular mass of 7,853 Dalton.
Rat Ccl3 expressed in E.coli
CAT# CSC-CTK0862-20 (20 μg); CAT# CSC-CTK0862-100 (100 μg)
Greater than 95% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.
Recombinant rat MIP-1 alpha is fully biologically active when compared to a standard. The activity is calculated by the ability to chemoattract rat peritoneal macrophages using a concentration of 60 ng/ml.
Less than 1 EU/μg.
Sterile-filtered, white, lyophilized (freeze-dried) powder. Recombinant MIP-1 alpha was lyophilized from a concentrated (1 mg/ml), sterile solution containing no additives.
Please centrifuge product briefly before opening vial. rRa MIP-1 alpha should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into water or other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rRa MIP-1 alpha should be used immediately or stored long- term in undiluted working aliquots at -20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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