Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine- rich secreted proteins which is termed the RELM family, and is also described as ADSF (Adipose Tissue-Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone). Human resistin contains 114 amino acids as a prepeptide, and its hydrophobic N-terminal 20 amino acid signal peptide is cleaved before its secretion. Resistin circulates in human blood as a dimeric protein consisting of two 94 amino acid polypeptides, which are disulfide-linked via Cys26. Resistin may be an important link between obesity and insulin resistance. Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. Studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression. Recombinant mouse Resistin is is a 13.7 kDa protein containing 93 amino acids of the mouse Resistin and 31 additional amino acids - the signal sequence of phage fd (21 aa) and the C-terminal fusion of the Flag-tag (10 aa).
Mouse Retn expressed in E.coli
CAT# CSC-CTK0773-25 (25 μg); CAT# CSC-CTK0773-125 (125 μg)
Greater than 95% as determined by SDS-PAGE analysis.
Sterile-filtered, white, lyophilized (freeze-dried) powder. Sterile-filtered and lyophilized from a 0. 5 mg/ml solution in 0.05 M acetate buffer (pH 4).
Please centrifuge product briefly before opening vial. For reconstitution, add 0.2 ml of 0.1 M acetate buffer (pH 4) and let the lyophilized pellet dissolve completely. For conversion into a higher pH value, we recommend intensive dilution by the relevant buffer to a concentration of 10 μg/ml. In higher concentrations the solubility of this antigen is limited. This solution can then be stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. It should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
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