RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted) is a member of the b (CC) family of chemokines. It is produced by synovial fibroblasts, monocytes, T lymphocytes, eosinophils, mast cells, platelets, epithelial cells, and some tumor cells. RANTES is a chemoattractant for monocytes, T cells, basophils, eosinophils, NK cells, dendritic cells and mast cells. In addition, RANTES induces activation of basophils, eosinophils and NK cells and it shows HIV-inhibitory effects. RANTES binds to CCR1, CCR3, CCR5, DARC and US28 receptors. Mouse RANTES cDNA encodes a highly basic 91 amino acid residue precursor with a 23 amino acid residue hydrophobic signal peptide that is cleaved to generate the 68 amino acid residue mature protein. The recombinant mature protein has a predicted molecular mass of 7.9 kDa.
Mouse Ccl5 expressed in E.coli
A DNA sequence encoding the mature mouse RANTES protein was expressed in E. coli.
CAT# CSC-CTK0037-25 (25 μg); CAT# CSC-CTK0037-125 (125 μg)
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity was determined by the ability to chemoattract 2 day cultured human monocytes and the ED50 for this effect was typically 0.03 – 0.1 mg/mL.
Endotoxin level less than 0.1 ng per 1 mg of the cytokine as determined by the LAL method.
Lyophilized from a sterile solution in 30% acetonitrile, 0.1% TFA containing 50 mg of bovine serum albumin per 1 mg of cytokine.
It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 25 mg/mL of the cytokine.
Storage & Stability
Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted mouse CCL5 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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