Neurotrophin-4 (NT-4) is a member of the nerve growth factor (NGF) family of neurotrophins. It binds the TrkB receptor and acts as a survival factor for sensory neurons, although it has also been shown to sensitize cortical neurons to cell death (for a review, see 1). Together with other NGF family members NT-3 and BDNF, NT-4 has been described as a survival factor for human embryonic stem cells (hESC). Bioactive NT-3 is predicted to be a non-covalently linked homodimer. Mature recombinant NT-3 has a predicted molecular mass of approximately 13.6 kDa, with 119 amino acids following cleavage of the signal peptide and proprotein. The amino acid sequence of mature NT-3 is identical in human, mouse and rat.
Human NTF4 expressed in Sf21 insect cells
The DNA sequence encoding the NT-3 prepropeptide3 was inserted in a baculovirus expression vector and expressed in Sf 21 insect cells.
CAT# CSC-CTK0075-5 (5 μg); CAT# CSC-CTK0075-25 (25 μg)
Sf21 insect cells
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
The ED50 of NT-4, as measured by its ability to stimulate proliferation of the TrkB-transfected cell line, BaF-TrkB-BD, is typically 5 - 15 ng/mL.
Endotoxin level is less than 1.0 EU/μg of NT-4, as determined by the LAL method.
Lyophilized from a sterile-filtered solution in 30% acetonitrile plus 0.1% TFA containing 50 μg of bovine serum albumin per 1 μg of NT-4.
It is recommended that a stock solution at a concentration of no less than 10 μg/mL be prepared in sterile phosphate buffered saline containing at least 0.1% human or bovine serum albumin.
Storage & Stability
The lyophilized sample is stable for up to 1 year at -20°C to -70°C. Reconstituted NT-4 can be stored under sterile conditions at 2 - 8°C for 1 month or at -20°C to -70°C for 3 months in a manual defrost freezer without detectable loss of acti vity. Avoid repeated freezing and thawing.
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