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ActoFactor™ Recombinant Human Interleukin 17E (IL-25, SF20; E. coli)

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Cat.No.
CSC-CTK0262
Description
IL-17E is a disulfide-linked homodimer of two 145 amino acid polypeptide chains. It belongs to the IL-17 family of structurally-related cytokines that share a highly conserved C-terminal region, but differ from one another in their N-terminal regions and in their distinct biological roles. The six known members of this family, IL-17A through IL-17F, are secreted as homodimers. IL-17E stimulated secretion of IL-8, and induces activation of the transcription factor NK-kB in cells that express the IL-17BR receptor. Recombinant human IL-17E is a 33.8 kDa disulfide-linked homodimer of two 145 amino acid polypeptide chains.
Organism
Human
Product Overview
Human IL25 expressed in E.coli
Molecular Mass
33.8 kDa
Size
CAT# CSC-CTK0262-10 (10 μg); CAT# CSC-CTK0262-50 (50 μg)
Expression System
E.coli
Purity
Greater than 98% as determined by SDS-PAGE and HPLC analysis.
Activity
Determined by its ability to induce IL-8 in human PBMCs using a concentration range of 10-100 ng/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized (freeze-dried), sterile powder. Lyophilizedfrom a sterile-filtered (0.2 micron filter) protein solution in 10 mM acetic acid.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-17E should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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