Interleukin 1 is a name that designates two proteins, IL-1a and IL-1b, that are the products of distinct genes but recognize the same cell surface receptors. The proteins are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17 kDa. IL-1a and IL-1b are potent pro-inflammatory cytokines that induce a wide variety of biological activities on different cell types. Recombinant human IL-1a contains 159 amino acids and predicted molecular mass of 18 kDa.
Human IL1A expressed in E.coli
A DNA sequence encoding human IL-1a was expressed in E. Coli.
CAT# CSC-CTK0070-2 (2 μg); CAT# CSC-CTK0070-10 (10 μg)
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
The ED50 as measured in a cell proliferation assay using the murine helper T cell line, D10.G4.1, is typically 3 - 7 pg/mL.
Endotoxin level is less than 0.1 ng per mg of the cytokine, as determined by the LAL method.
Lyophilized from a sterile filtered solution in 20 mM Tris, pH 8.3, containing 50 mg of bovine serum albumin per 1 mg of cytokine.
It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin is added to the vial to prepare a stock solution of no less than 1 mg/mL of the cytokine.
Storage & Stability
The lyophilized powder is stable for greater than six months at -20°C to -70°C. Reconstituted IL-1b can be stored under sterile conditions at 2o to 4 °C for one month or at -20°to -70°C for three months without detectable loss of activity. Avoid repeated freezing and thawing.
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