IGF-1R (Insulin-like Growth Factor-1 Receptor) consists of alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The alpha- subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity. On SDS-PAGE (Commassie Blue-stained gel), the purified recombinant human IGF-IR protein (a.a. 1509-1663) shows a band at 43 kDa.
Human IGF1R expressed in E.coli
CAT# CSC-CTK0200-5 (5 μg); CAT# CSC-CTK0200-25 (25 μg)
Greater than 98% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.
Less than 1 EU/μg.
Sterile-filtered, liquid. 100 μg/ml in 50 mM Tris-Acetate (pH7.5), 1 mM EDTA and 20% glycerol.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be stored long-term in undiluted working aliquots at –20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles..
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