Recombinant human Eotaxin produced in E. coli is a single, non-glycosylated polypeptide chain of 74 amino acids and a molecular mass of 8,346 Dalton.
Human CCL11 expressed in E.coli
CAT# CSC-CTK0439-20 (20 μg); CAT# CSC-CTK0439-100 (100 μg)
Greater than 98% as determined by SDS-PAGE and RP-HPLC analysis.
The activity is determined by the chemoattraction of human PBE (peripheral blood eosinophils). It produced a significant increase at a concentration of 100 ng/ml compared to the medium control.
Less than 1 EU/μg.
White, lyophilized (freeze-dried) powder. Lyophiliz ed from a sterile-filtered protein solution containing no additives.
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu Eotaxin should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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