Activin A is a member of the transforming growth factor β (TGF-β) superfamily. It has a wide range of biological activities including driving endoderm and mesoderm induction and regulating the differentiation of many cell types. Activin A has also been shown to support maintenance of human embryonic stem cells in the undifferentiated state. It shares receptors with the ligand nodal and activates the same downstream SMAD pathway as TGF-β. Recombinant human mature Activin A is a disulfide-linked homodimer of two 116 amino acid residue βA subunits with a molecular weight of 13 kDa.
Human Activin A expressed in CHO cells
Recombinant protein expressed in Chinese Hamster Ovary (CHO) cells.
CAT# CSC-CTK0086-10 (10 μg); CAT# CSC-CTK0086-50 (50 μg)
Greater than 95% as determined by SDS-PAGE and visualized by silver stain
The ED50 of Activin A, as measured by its ability to induce hemoglobin expression in K562 cells, is typically 0.2 - 1.2 ng/mL.
Endotoxin level is less than 1.0 EU/μg of Activin A as assayed by the Limulus Amoebocyte Lysate (LAL) method.
Lyophilized from a 0.2 μm filtered solution in Acetonitrile plus TFA with BSA as a carrier protein.
Reconstitute to a concentration of 50 μg/mL with sterile phosphate buffered saline (PBS).
Storage & Stability
Product stable at -20°C for 12 months from date of receipt. Once reconstituted, product can be aliquoted. Reconstituted product is stable at 2 - 8°C for one month or at -20°C for 3 months. Avoid additional freeze-thaw cycles.
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